Enhancing the Upstream Performance of Adeno-Associated Virus (AAV) Vector Manufacturing via Multivariate Data Analysis

In the race to deliver gene therapies to patients faster, every step of the manufacturing process counts. Viralgen is applying multivariate data analysis (MVDA) to boost upstream AAV production, uncovering key insights that improve process performance and consistency.

Presented in our recent research, “Enhancing the Upstream Performance of Adeno-Associated Virus (AAV) Vector Manufacturing via Multivariate Data Analysis,” the study identifies the process parameters most closely linked to productivity—and how to act on them.

Key Findings:

• Higher viable cell density at transfection was negatively correlated with productivity.
• Glucose concentration and pH showed strong correlations with AAV yield.
• The model was successfully deployed to predict batch performance early in production, helping the team take proactive corrective actions.

Download the PDF to learn more.